BMEM Human Primed Pluripotent is a highly optimized, chemically defined, animal origin-free (AOF) basal medium and supplement combination for the maintenance and expansion of human induced pluripotent stem cells (hiPSCs) in adherent culture.•Optimized Formulation: Features a new Primed Supplement designed to ease the transition of hiPSCs from legacy media (e.g., mTeSR1™), resolving a key challenge of chemically defined systems.•Enhanced Performance: Promotes increased cell proliferation and upregulation of core pluripotency factors.•Protocol Friendly: Supports weekend-free and no-change feeding schedules (from original full text).•Broad Compatibility: Works with diverse matrices (Matrigel, Geltrex™, Vitronectin) and passaging methods (TrypLE™, ReLeSR™, Versene).•Quality & Stability: Supplement is optimized for stability (allowing room temperature shipment). Each lot is performance tested on hiPSCs and confirmed sterile.